Journal: Molecular Neurobiology

Article Title: SirT2 Inhibition is Associated with Improvements in Depression-like Behavior and Memory Impairment in Olfactory Bulbectomized Mice

doi: 10.1007/s12035-026-05868-y

Figure Lengend Snippet: Effects of AK-7 on the SirT2/Ac-FoxO1/PPARγ signaling pathway in the PFC of OBX mice. A - F Expression levels of SirT2 ( A ), Ac-FoxO1 ( B ), p-FoxO1 ( C ), PPARγ ( D ), p-AKT (Thr308) ( E ), and p-AKT (Ser473) ( F ) in the PFC of OBX mice. G: Representative immunofluorescence images showing spatial overlap of SirT2, Ac-FoxO1, and PPARγ with the microglial marker Iba1 in the PFC of OBX mice treated with AK-7. H: Proposed mechanistic model of AK-7 action. A – F One-way ANOVA; A F (2, 24) = 8.965, p = 0.0012; B F (2, 24) = 7.719, p = 0.0026; C F (2, 24) = 5.336, p = 0.0121; D F (2, 24) = 8.487, p = 0.0016; E F (2, 18) = 6.321, p = 0.0083; F F (2, 18) = 13.48, p = 0.0003. All data are presented as mean ± SEM. *: p < 0.05, **: p < 0.01 vs. sham + vehicle group. #: p < 0.05, ##: p < 0.01 vs. OBX + vehicle group

Article Snippet: Membranes were blocked for 30 min in Tris-buffered saline containing 0.01% Tween-20 (TBST) supplemented with 5% skim milk and subsequently incubated overnight at 4 °C with primary antibodies against SirT1 (1:2000; Millipore, Billerica, MA, USA; 07–131), SirT2 (1:2000; Cell Signaling Technology [CST], Danvers, MA, USA; #12650), SirT3 (1:1000; CST; #5490), SirT4 (1:2000; Proteintech, Rosemont, IL, USA; 66543–1-Ig), SirT5 (1:1000; CST; #8782), SirT6 (1:1000; CST; #12486), SirT7 (1:1000; CST; #5360), ΔFosB (1:500; CST; #14695), HRD1 (1:500; Proteintech; 13473–1-AP), acetyl-FoxO1 (Lys294; 1:200; Invitrogen, Waltham, MA, USA; PA5-104560), phospho-FoxO1 (Ser256; 1:500; CST; #9461), total FoxO1 (1:500; CST; #2880), PPARγ (1:500; CST; #2435), phospho-AKT (Thr308; 1:200; CST; #13038), phospho-AKT (Ser473; 1:1000; CST; #4060), total AKT (1:1000; CST; #4691), CD86 (1:500; Proteintech; 13395–1-AP), inducible nitric oxide synthase (iNOS; 1:50000; Proteintech; 18985–1-AP), interleukin-1β (IL-1β; 1:500; Proteintech; 26048–1-AP), tumor necrosis factor-α (TNF-α; 1:100; Proteintech; 17590–1-AP), arginase-1 (1:500; Proteintech; 66129–1-Ig), transforming growth factor-β (TGF-β; 1:500; Proteintech; 21898–1-AP), myelin basic protein (MBP; 1:1000; CST; #78896), myelin-associated glycoprotein (MAG; 1:1000; CST; #9043), cyclicnucleotide phosphodiesterase (CNPase; 1:1000; CST; #5664), contactin-associated protein (Caspr; 1:1000; Abcam, Cambridge, UK; ab246501), and β-actin (1:1000; Santa Cruz Biotechnology, Dallas, TX, USA; SC-47778).

Techniques: Expressing, Immunofluorescence, Marker